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CoraLite® Plus 488 Rat IgG1 Isotype Control (HRPN)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HRPN

Cat no : CL488-65212

Print datasheet

Synonyms

IgG1, IgG1 Isotype Control, Rat IgG1 Isotype Control

验证数据展示

  • FC experiment of mouse splenocytes using CL488-65212

    FC experiment of mouse splenocytes using CL488-65212

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and 5 ul CoraLite® Plus 488-conjugated Anti-Mouse CD25 (CL488-65137, Clone: PC61.5). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL488-65212

    FC experiment of mouse splenocytes using CL488-65212

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and CoraLite® Plus 488-conjugated Rat IgG1 Isotype Control (CL488-65212, Clone: HRPN). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65212-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65212 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name IgG1 Isotype Control
Synonyms IgG1, IgG1 Isotype Control, Rat IgG1 Isotype Control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2919275
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The HRPN immunoglobulin is useful as an isotype-matched control. The HRPN immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IgG1 Isotype Control antibody CL488-65212Download protocol
Standard Protocols
Click here to view our Standard Protocols