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FITC Plus Rat IgG1 Isotype Control (HRPN)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

HRPN

Cat no : FITC-65212

Print datasheet

Synonyms

Rat IgG1 Isotype Control, IgG1

验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using FITC-65212

    FC experiment of C57BL/6 mouse splenocytes using FITC-65212

    1X10^6 anti-CD3/CD28 treated (2 days) C57BL/6 mouse splenocytes were surface stained with 0.25 ug FITC Anti-Rat IgG1 Isotype Control (FITC-65212, Clone: HRPN) (red), or 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5) (green) or unstained (blue). Cells were not fixed.

经过测试的应用

Positive FC detected in

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65212 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name IgG1 Isotype Control
Synonyms Rat IgG1 Isotype Control, IgG1
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883826
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The HRPN immunoglobulin is useful as an isotype-matched control. The HRPN immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for FITC Plus IgG1 Isotype Control antibody FITC-65212Download protocol
Standard Protocols
Click here to view our Standard Protocols