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Integrin beta 3 Recombinant antibody, PBS Only

Integrin beta 3 Recombinant Antibody for IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230292D5

Cat No : 83053-4-PBS

Print datasheet

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

验证数据展示

  • IF Staining of HUVEC using 83053-4-RR (same clone as 83053-4-PBS)

    IF Staining of HUVEC using 83053-4-RR (same clone as 83053-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Integrin beta-3 antibody (83053-4-RR, Clone: 230292D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83053-4-PBS in a different storage buffer formulation.

  • FC experiment of HUVEC using 83053-4-RR (same clone as 83053-4-PBS)

    FC experiment of HUVEC using 83053-4-RR (same clone as 83053-4-PBS)

    1x10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human Integrin beta-3 (83053-4-RR, Clone:230292D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83053-4-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.

产品信息

83053-4-PBS targets Integrin beta 3 in IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications IF, FC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Synonyms CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3
Calculated Molecular Weight 87 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC127667
Gene Symbol ITGB3
Gene ID (NCBI) 3690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05106
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.