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Integrin beta 3 Recombinant antibody

Integrin beta 3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230292D5

Cat no : 83053-4-RR

Print datasheet

Synonyms

ITGB3, Integrin beta-3, GPIIIa, GP3A, CD61

验证数据展示

  • IF Staining of HUVEC using 83053-4-RR

    IF Staining of HUVEC using 83053-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Integrin beta-3 antibody (83053-4-RR, Clone: 230292D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HUVEC using 83053-4-RR

    FC experiment of HUVEC using 83053-4-RR

    1x10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human Integrin beta-3 (83053-4-RR, Clone:230292D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83053-4-RR targets Integrin beta 3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Synonyms ITGB3, Integrin beta-3, GPIIIa, GP3A, CD61
Calculated Molecular Weight 87 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC127667
Gene Symbol Integrin beta 3
Gene ID (NCBI) 3690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

实验方案

Product Specific Protocols
IF protocol for Integrin beta 3 antibody 83053-4-RRDownload protocol
FC protocol for Integrin beta 3 antibody 83053-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols