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CoraLite® Plus 488-conjugated Integrin beta 3 Recombinant antibody

Integrin beta 3 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230292D5

Cat No : CL488-83053-4

Print datasheet

Synonyms

Integrin beta 3, ITGB3, Integrin beta-3, 230292D5, CD61, GP3A, GPIIIa

验证数据展示

  • FC experiment of HUVEC using CL488-83053-4

    FC experiment of HUVEC using CL488-83053-4

    1x10^6 HUVEC cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Integrin Beta-3 Recombinant Antibody (CL488-83053-4, Clone:230292D5)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83053-4-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83053-4 targets Integrin beta 3 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Synonyms Integrin beta 3, ITGB3, Integrin beta-3, 230292D5, CD61, GP3A, GPIIIa
Calculated Molecular Weight 87 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC127667
Gene Symbol Integrin beta 3
Gene ID (NCBI) 3690
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05106
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Integrin beta 3 antibody CL488-83053-4Download protocol
Standard Protocols
Click here to view our Standard Protocols