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JTV1 Monoclonal antibody

JTV1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66848-1-Ig

Print datasheet

Synonyms

PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, AIMP2

验证数据展示

  • WB analysis using 66848-1-Ig

    WB analysis using 66848-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 66848-1-Ig

    IHC staining of mouse testis using 66848-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AIMP2/JTV1 antibody included
  • FC experiment of HepG2 using 66848-1-Ig

    FC experiment of HepG2 using 66848-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug JTV1 Monoclonal antibody (66848-1-Ig, Clone:1D6B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, HL-60 cells. HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AIMP2/JTV1 Ready-To-Use IHC Kit. AIMP2/JTV1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66848-1-Ig targets JTV1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen JTV1 fusion protein Ag0675 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name JTV1 gene
Synonyms PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, AIMP2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC002853
Gene Symbol JTV1
Gene ID (NCBI) 7965
RRIDAB_2882188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13155
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

实验方案

Product Specific Protocols
WB protocol for JTV1 antibody 66848-1-IgDownload protocol
IHC protocol for JTV1 antibody 66848-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols