• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

JTV1 Monoclonal antibody, PBS Only

JTV1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66848-1-PBS

Print datasheet

Synonyms

PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, AIMP2

验证数据展示

  • WB analysis using 66848-1-Ig (same clone as 66848-1-PBS)

    WB analysis using 66848-1-Ig (same clone as 66848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66848-1-Ig (same clone as 66848-1-PBS)

    IHC staining of mouse testis using 66848-1-Ig (same clone as 66848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIMP2/JTV1 antibody included
  • FC experiment of HepG2 using 66848-1-Ig (same clone as 66848-1-PBS)

    FC experiment of HepG2 using 66848-1-Ig (same clone as 66848-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug JTV1 Monoclonal antibody (66848-1-Ig, Clone:1D6B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AIMP2/JTV1 Ready-To-Use IHC Kit. AIMP2/JTV1 primary antibody included.

产品信息

66848-1-PBS targets JTV1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen JTV1 fusion protein Ag0675 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name JTV1 gene
Synonyms PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, AIMP2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC002853
Gene Symbol JTV1
Gene ID (NCBI) 7965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13155
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.