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验证数据展示

  • IF Staining of HeLa using CL488-83134-6

    IF Staining of HeLa using CL488-83134-6

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 JUND antibody (CL488-83134-6, Clone: 230457A4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83134-6

    FC experiment of HeLa using CL488-83134-6

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 JUND Recombinant Antibody (CL488-83134-6, Clone:230457A4)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated JUND Recombinant antibody

JUND Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83134-6
CloneNo. 230457A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Transcription factor jun D, jun D proto oncogene, AP 1, 230457A4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy JUND Ready-To-Use IHC Kit. JUND primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83134-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83134-6 targets JUND in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name jun D proto-oncogene
Synonyms Transcription factor jun D, jun D proto oncogene, AP 1, 230457A4
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35-45 kDa
GenBank Accession NumberNM_005354
Gene Symbol JUND
Gene ID (NCBI) 3727
RRIDAB_3673199
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17535
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 JUND antibody CL488-83134-6Download protocol
Standard Protocols
Click here to view our Standard Protocols