• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68613-1-Ig (same clone as 68613-1-PBS)

    WB analysis using 68613-1-Ig (same clone as 68613-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68613-1-Ig (KANK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68613-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68613-1-Ig (same clone as 68613-1-PBS)

    IF Staining of HepG2 using 68613-1-Ig (same clone as 68613-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KANK1 antibody (68613-1-Ig, Clone: 2D3G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68613-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68613-1-Ig (same clone as 68613-1-PBS)

    FC experiment of HepG2 using 68613-1-Ig (same clone as 68613-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KANK1 Monoclonal antibody (68613-1-Ig, Clone:2D3G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68613-1-PBS in a different storage buffer formulation.

KANK1 Monoclonal antibody, PBS Only

KANK1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68613-1-PBS

产品说明书

CloneNo. 2D3G8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy KANK1 Ready-To-Use IHC Kit. KANK1 primary antibody included.

产品信息

68613-1-PBS targets KANK1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 KANK1 fusion protein Ag31440 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KN motif and ankyrin repeat domains 1
别名 KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15
计算分子量147KD
观测分子量130-200 kDa
GenBank蛋白编号NM_015158
基因名称 KANK1
Gene ID (NCBI) 23189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14678
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).