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验证数据展示

  • WB analysis using 84669-2-RR (same clone as 84669-2-PBS)

    WB analysis using 84669-2-RR (same clone as 84669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • WB analysis using 84669-2-RR (same clone as 84669-2-PBS)

    WB analysis using 84669-2-RR (same clone as 84669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84669-2-RR (same clone as 84669-2-PBS)

    IF Staining of MCF-7 using 84669-2-RR (same clone as 84669-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KAT2A/GCN5 antibody (84669-2-RR, Clone: 242113C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 84669-2-RR (same clone as 84669-2-PBS)

    FC experiment of HEK-293 using 84669-2-RR (same clone as 84669-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KAT2A/GCN5 Recombinant antibody (84669-2-RR, Clone:242113C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01463-1

    Cytometric bead array standard curve of MP01463-1

    Cytometric bead array standard curve of MP01463-1, KAT2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84669-2-PBS. Detection antibody: 84669-3-PBS. Standard:Ag29005. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84669-2-RR

    Affinity and Kinetic Characterization of 84669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84669-2-RR against Human KAT2A/GCN5 were performed. The affinity constant is 0.252 nM.

KAT2A/GCN5 Recombinant antibody, PBS Only (Capture)

KAT2A/GCN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84669-2-PBS

产品说明书

CloneNo. 242113C7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84669-2-PBS targets KAT2A/GCN5 as part of a matched antibody pair:

MP01463-1: 84669-2-PBS capture and 84669-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 KAT2A/GCN5 fusion protein Ag29005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 K(lysine) acetyltransferase 2A
别名 General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-
计算分子量 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC032743
基因名称 KAT2A
Gene ID (NCBI) 2648
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92830
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.