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验证数据展示

  • WB analysis using 84669-2-RR

    WB analysis using 84669-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 84669-2-RR

    WB analysis using 84669-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84669-2-RR

    IF Staining of MCF-7 using 84669-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KAT2A/GCN5 antibody (84669-2-RR, Clone: 242113C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 84669-2-RR

    FC experiment of HEK-293 using 84669-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KAT2A/GCN5 Recombinant antibody (84669-2-RR, Clone:242113C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84669-2-RR

    Affinity and Kinetic Characterization of 84669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84669-2-RR against Human KAT2A/GCN5 were performed. The affinity constant is 0.252 nM.

KAT2A/GCN5 Recombinant antibody

KAT2A/GCN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84669-2-RR

产品说明书

CloneNo. 242113C7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, LNCaP cells, HSC-T6 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84669-2-RR targets KAT2A/GCN5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 KAT2A/GCN5 fusion protein Ag29005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 K(lysine) acetyltransferase 2A
别名 General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-
计算分子量 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC032743
基因名称 KAT2A
Gene ID (NCBI) 2648
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

实验方案

Product Specific Protocols
WB protocol for KAT2A/GCN5 antibody 84669-2-RRDownload protocol
IF protocol for KAT2A/GCN5 antibody 84669-2-RRDownload protocol
FC protocol for KAT2A/GCN5 antibody 84669-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols