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CoraLite® Plus 488-conjugated KDM3A,JMJD1A Recombinant antibody

KDM3A,JMJD1A Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240532E2

Cat No : CL488-83571-6

Print datasheet

Synonyms

验证数据展示

  • IF Staining of HepG2 using CL488-83571-6

    IF Staining of HepG2 using CL488-83571-6

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 KDM3A,JMJD1A antibody (CL488-83571-6, Clone: 240532E2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KDM3A Ready-To-Use IHC Kit. KDM3A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83571-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83571-6 is KDM3A,JMJD1A Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen KDM3A,JMJD1A fusion protein Ag28687 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lysine (K)-specific demethylase 3A
Synonyms
Calculated Molecular Weight 150 kDa
GenBank Accession NumberBC038297
Gene Symbol KDM3A/JMJD1A
Gene ID (NCBI) 55818
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4C1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KDM3A,JMJD1A antibody CL488-83571-6Download protocol
Standard Protocols
Click here to view our Standard Protocols