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验证数据展示

  • IF Staining of HepG2 using CL488-83571-6

    IF Staining of HepG2 using CL488-83571-6

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 KDM3A,JMJD1A antibody (CL488-83571-6, Clone: 240532E2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated KDM3A/JMJD1A Recombinant antibody

KDM3A/JMJD1A Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83571-6

产品说明书

CloneNo. 240532E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KDM3A Ready-To-Use IHC Kit. KDM3A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83571-6-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83571-6 targets KDM3A/JMJD1A in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 KDM3A/JMJD1A fusion protein Ag28687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysine (K)-specific demethylase 3A
别名 KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A
计算分子量 150 kDa
GenBank蛋白编号BC038297
基因名称 KDM3A/JMJD1A
Gene ID (NCBI) 55818
RRIDAB_3673274
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4C1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KDM3A/JMJD1A antibody CL488-83571-6Download protocol
Standard Protocols
Click here to view our Standard Protocols