CoraLite® Plus 488-conjugated KDM3A/JMJD1A Recombinant antibody
KDM3A/JMJD1A Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240532E2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy KDM3A Ready-To-Use IHC Kit. KDM3A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83571-6-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83571-6 is KDM3A/JMJD1A Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | KDM3A/JMJD1A fusion protein Ag28687 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | lysine (K)-specific demethylase 3A |
| Synonyms | KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A |
| Calculated Molecular Weight | 150 kDa |
| GenBank Accession Number | BC038297 |
| Gene Symbol | KDM3A/JMJD1A |
| Gene ID (NCBI) | 55818 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4C1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 KDM3A/JMJD1A antibody CL488-83571-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |