验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, PC-3 cells, HepG2 cells, K-562 cells, HEK-293T cells, rat testis tissue |
| Positive FC (Intra) detected in | PC-3 cells, U-251 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84217-4-RR targets KLHDC3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, FC (Intra), ELISA Application Description |
| Tested Reactivity | human, rat |
| Immunogen | KLHDC3 fusion protein Ag4879 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | kelch domain containing 3 |
| Synonyms | PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5 |
| Calculated Molecular Weight | 382 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC041793 |
| Gene Symbol | KLHDC3 |
| Gene ID (NCBI) | 116138 |
| RRID | AB_3671773 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BQ90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLHDC3 antibody 84217-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
