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验证数据展示

  • IF Staining of HepG2 using CL488-67892

    IF Staining of HepG2 using CL488-67892

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 KPNA3 antibody (CL488-67892, Clone: 1D3D2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KPNA3 Monoclonal antibody

KPNA3 Monoclonal Antibody for IF/ICC
Cat No. CL488-67892
CloneNo. 1D3D2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

IF/ICC

Synonyms

hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA3 Ready-To-Use IHC Kit. KPNA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67892-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67892 targets KPNA3 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, rat, mouse
Immunogen KPNA3 fusion protein Ag27767 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name karyopherin alpha 3 (importin alpha 4)
Synonyms hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4
Calculated Molecular Weight 58 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC017355
Gene Symbol KPNA3
Gene ID (NCBI) 3839
RRIDAB_2934583
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00505
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

karyopherin subunit alpha 3 (KPNA3) is a member of the importin alpha family which can transport molecules between the mucleus and cytoplasm. It is ubiquitous expressed in brain,testis and esophagus. KPNA3 is associated with many severe diseases such as hepatocellular Carcinoma (PMID: 31417635) , schizophrenia, major depression and opiate dependence (PMID: 22960338) . The molecular weight of KPNA3 is about 58 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KPNA3 antibody CL488-67892Download protocol
Standard Protocols
Click here to view our Standard Protocols