• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated KPNA4 Recombinant antibody

KPNA4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230098H1

Cat No : CL488-82898-2

Print datasheet

Synonyms

Importin alpha Q1, Importin subunit alpha 4, IPOA3, Karyopherin subunit alpha 4, KPNA4, QIP1, SRP3

验证数据展示

  • IF Staining of U2OS using CL488-82898-2

    IF Staining of U2OS using CL488-82898-2

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 KPNA4 antibody (CL488-82898-2, Clone: 230098H1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 82898-2-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82898-2 targets KPNA4 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen KPNA4 fusion protein Ag3133 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name karyopherin alpha 4 (importin alpha 3)
Synonyms Importin alpha Q1, Importin subunit alpha 4, IPOA3, Karyopherin subunit alpha 4, KPNA4, QIP1, SRP3
Calculated Molecular Weight 521 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC034493
Gene Symbol KPNA4
Gene ID (NCBI) 3840
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00629
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KPNA4 antibody CL488-82898-2Download protocol
Standard Protocols
Click here to view our Standard Protocols