• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KRAS-2A-specific Polyclonal antibody, PBS Only

KRAS-2A-specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 16156-1-PBS

Print datasheet

Synonyms

KRAS, KRAS4A, KRAS4B, pan RAS, GTPase KRas

验证数据展示

  • WB analysis of NIH/3T3 using 16156-1-AP (same clone as 16156-1-PBS)

    WB analysis of NIH/3T3 using 16156-1-AP (same clone as 16156-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • WB analysis using 16156-1-AP (same clone as 16156-1-PBS)

    WB analysis using 16156-1-AP (same clone as 16156-1-PBS)

    Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 16156-1-AP (same clone as 16156-1-PBS)

    FC experiment of HeLa using 16156-1-AP (same clone as 16156-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS-2A-specific (16156-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 16156-1-AP (same clone as 16156-1-PBS)

    IHC staining of human stomach using 16156-1-AP (same clone as 16156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16156-1-AP (same clone as 16156-1-PBS)

    IF Staining of HeLa using 16156-1-AP (same clone as 16156-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KRAS-2A-specific antibody (16156-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 16156-1-PBS in a different storage buffer formulation.

产品信息

16156-1-PBS targets KRAS-2A-specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS, KRAS4A, KRAS4B, pan RAS, GTPase KRas
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberNM_033360
Gene Symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .