验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, mouse brain tissue, Recombinant protein |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| FC | See 1 publications below |
产品信息
16155-1-AP targets KRAS-4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Synonyms | KRAS-4B-specific, KRAS, pan RAS, GTPase KRas, EC:3.6.5.2 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | NM_004985 |
| Gene Symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2134119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4B, which is also named as KRAS-2B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KRAS-4B antibody 16155-1-AP | Download protocol |
| IHC protocol for KRAS-4B antibody 16155-1-AP | Download protocol |
| IF protocol for KRAS-4B antibody 16155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors. | ||
Nat Biomed Eng Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. | ||
Cell Chem Biol Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System. | ||
Sci Signal Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. | ||
Life Sci Alliance A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95 |
