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  • Featured Product
  • KD/KO Validated

KRAS-2B Polyclonal antibody

KRAS-2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16155-1-AP

Print datasheet

Synonyms

KRAS-2B-specific, KRAS, KRAS4A, KRAS4B, pan RAS

验证数据展示

  • WB analysis of HeLa using 16155-1-AP

    WB analysis of HeLa using 16155-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16155-1-AP

    WB analysis using 16155-1-AP

    Recombinant proteins(ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS))were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16155-1-AP

    WB analysis of HEK-293 using 16155-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16155-1-AP

    IHC staining of human colon cancer using 16155-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16155-1-AP

    IHC staining of human colon cancer using 16155-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16155-1-AP

    IF Staining of HeLa using 16155-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KRAS-2B-specific antibody (16155-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, mouse brain tissue, Recombinant protein
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16155-1-AP targets KRAS-2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS-2B-specific, KRAS, KRAS4A, KRAS4B, pan RAS
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberNM_004985
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2134119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2B.

实验方案

Product Specific Protocols
WB protocol for KRAS-2B antibody 16155-1-APDownload protocol
IHC protocol for KRAS-2B antibody 16155-1-APDownload protocol
IF protocol for KRAS-2B antibody 16155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nat Biotechnol

Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.

Authors - Christopher W Davies
View Article
humanFC

Nat Biomed Eng

Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.

Authors - Mahmoud Labib
View Article
humanWB

Cancer Res

Targeting eIF4A-Dependent Translation of KRAS Signaling Molecules.

Authors - Kamini Singh
View Article
humanWB

Cell Chem Biol

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Authors - Sascha Röth
View Article
humanWB

Sci Signal

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Authors - Andrew M Waters
View Article
humanWB

Life Sci Alliance

A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95

Authors - Kristen M Rehl
View Article