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- KD/KO Validated
pan Ras Monoclonal antibody
pan Ras Monoclonal Antibody for FC (Intra), IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit, chicken
Applications
FC (Intra), IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4H4G7
验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, pig brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60309-1-Ig targets pan Ras in WB, IP, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| Tested Applications | FC (Intra), IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | pan Ras fusion protein Ag2700 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Synonyms | C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2 |
| Calculated Molecular Weight | 188 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC013572 |
| Gene Symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2881422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| IHC protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| IP protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| FC protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells. | ||
Cancer Res KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.
| ||
Exp Mol Med Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer. | ||
J Cell Mol Med DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells. | ||
Mol Pharm Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer | ||
Oncol Rep EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells. |
