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- KD/KO Validated
CoraLite® Plus 488-conjugated pan Ras Monoclonal antibody
pan Ras Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4H4G7
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 60309-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60309 targets pan Ras in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | pan Ras fusion protein Ag2700 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Synonyms | KRAS, KRAS4A, KRAS4B, EC:3.6.5.2, c-K-ras |
| Calculated Molecular Weight | 188 aa, 21 kDa |
| GenBank Accession Number | BC013572 |
| Gene Symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2919248 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01116 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 pan Ras antibody CL488-60309 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
