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验证数据展示

  • WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

    WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

    WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 60309-1-Ig (same clone as 60309-1-PBS)

    IP experiment of mouse brain using 60309-1-Ig (same clone as 60309-1-PBS)

    IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60309-1-Ig (same clone as 60309-1-PBS)

    FC experiment of HeLa using 60309-1-Ig (same clone as 60309-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human pan Ras (60309-1-Ig, Clone:4H4G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    IHC staining of human ovary cancer using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60309-1-Ig (pan Ras antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

    IF Staining of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using pan Ras antibody (60309-1-Ig, Clone: 4H4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50398-1

    Cytometric bead array standard curve of MP50398-1

    Cytometric bead array standard curve of MP50398-1, KRAS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60309-2-PBS. Detection antibody: 60309-1-PBS. Standard:Ag2700. Range: 0.781-12.5 ng/mL.

  • Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

  • Featured Product
  • KD/KO验证

pan Ras Monoclonal antibody, PBS Only (Capture/Detector)

pan Ras Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Cat No. 60309-1-PBS

产品说明书

CloneNo. 4H4G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

别名

KRAS, EC:3.6.5.2, c-K-ras, c-Ki-ras, c Ki ras

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60309-1-PBS targets pan Ras as part of a matched antibody pair:

MP50398-1: 60309-2-PBS capture and 60309-1-PBS detection (validated in Cytometric bead array)

MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原 pan Ras fusion protein Ag2700 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
别名 KRAS, EC:3.6.5.2, c-K-ras, c-Ki-ras, c Ki ras
计算分子量 188 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC013572
基因名称 KRAS
Gene ID (NCBI) 3845
RRIDAB_2881422
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01116
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.