验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells, MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82972 targets Cytokeratin 1-specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | keratin 1 |
| Synonyms | KRT1-specific, Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | NM_006121 |
| Gene Symbol | KRT1 |
| Gene ID (NCBI) | 3848 |
| RRID | AB_3673159 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04264 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Cytokeratin 1-specific antibody CL488-82972 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |