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验证数据展示

  • IHC staining of mouse brain using Biotin-67115

    IHC staining of mouse brain using Biotin-67115

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with L1CAM antibody included

Biotin-conjugated L1CAM Monoclonal antibody

L1CAM Monoclonal Antibody for IHC
Cat No. Biotin-67115

Print datasheet

CloneNo. 3H7B9

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IHC

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-67115 targets L1CAM in IHC applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications IHC Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen L1CAM fusion protein Ag17706 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1
Calculated Molecular Weight 1257 aa, 140 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
RRIDAB_2918949
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32004
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
IHC protocol for Biotin L1CAM antibody Biotin-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols