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验证数据展示

  • IHC staining of mouse brain using Biotin-67115

    IHC staining of mouse brain using Biotin-67115

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with L1CAM antibody included

Biotin-conjugated L1CAM Monoclonal antibody

L1CAM Monoclonal Antibody for IHC
Cat No. Biotin-67115

产品说明书

CloneNo. 3H7B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Mouse, Rat

应用

IHC

别名

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-67115 targets L1CAM in IHC applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 IHC Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原 L1CAM fusion protein Ag17706 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1
计算分子量 1257 aa, 140 kDa
观测分子量 220 kDa
GenBank蛋白编号BC126229
基因名称 L1CAM
Gene ID (NCBI) 3897
RRIDAB_2918949
偶联类型 Biotin
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32004
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
IHC protocol for Biotin L1CAM antibody Biotin-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols