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CoraLite®594-conjugated L1CAM Monoclonal antibody

L1CAM Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H7B9

Cat no : CL594-67115

Print datasheet

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

验证数据展示

  • IF Staining of mouse brain using CL594-67115

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-67115

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67115-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67115 targets L1CAM in IF-P applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen L1CAM fusion protein Ag17706 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1
Calculated Molecular Weight 1257 aa, 140 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
RRIDAB_2920087
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32004
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
IF protocol for CL594 L1CAM antibody CL594-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols