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验证数据展示

  • WB analysis using CL750-82946

    WB analysis using CL750-82946

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82946 (LAMB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-82946

    FC experiment of HepG2 using CL750-82946

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Coralite® Plus 750 Lamb2 Recombinant Antibody (CL750-82946, Clone:230143E4)(red), or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 750-conjugated LAMB2 Recombinant antibody

LAMB2 Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82946

产品说明书

CloneNo. 230143E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra)

别名

LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82946-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82946 targets LAMB2 in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 LAMB2 fusion protein Ag33468 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 laminin, beta 2 (laminin S)
别名 LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta
计算分子量196 kDa
观测分子量195-200 kDa
GenBank蛋白编号NM_002292.3
基因名称 LAMB2
Gene ID (NCBI) 3913
RRIDAB_3673749
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55268
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 LAMB2 antibody CL750-82946Download protocol
Standard Protocols
Click here to view our Standard Protocols