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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84522-6-RR (same clone as 84522-6-PBS)

    WB analysis using 84522-6-RR (same clone as 84522-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84522-6-RR (LBR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84522-6-RR (same clone as 84522-6-PBS)

    FC experiment of HepG2 using 84522-6-RR (same clone as 84522-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84522-6-RR (same clone as 84522-6-PBS)

    FC experiment of HeLa using 84522-6-RR (same clone as 84522-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

LBR Recombinant antibody, PBS Only

LBR Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84522-6-PBS

产品说明书

CloneNo. 241864H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LBR Ready-To-Use IHC Kit. LBR primary antibody included.

产品信息

84522-6-PBS targets LBR in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LBR fusion protein Ag3088 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lamin B receptor
别名 Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1
计算分子量 615 aa, 71 kDa
观测分子量58 kDa
GenBank蛋白编号BC020079
基因名称 LBR
Gene ID (NCBI) 3930
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14739
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).