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验证数据展示

  • WB analysis using 84522-6-RR

    WB analysis using 84522-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84522-6-RR (LBR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84522-6-RR

    FC experiment of HepG2 using 84522-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 84522-6-RR

    FC experiment of HeLa using 84522-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LBR Recombinant antibody

LBR Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84522-6-RR

Print datasheet

CloneNo. 241864H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, C2C12 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LBR Ready-To-Use IHC Kit. LBR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84522-6-RR targets LBR in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LBR fusion protein Ag3088 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin B receptor
Synonyms Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1
Calculated Molecular Weight 615 aa, 71 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020079
Gene Symbol LBR
Gene ID (NCBI) 3930
RRIDAB_3672029
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

实验方案

Product Specific Protocols
WB protocol for LBR antibody 84522-6-RRDownload protocol
FC protocol for LBR antibody 84522-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols