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验证数据展示

  • WB analysis using 84522-6-RR

    WB analysis using 84522-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84522-6-RR (LBR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84522-6-RR

    FC experiment of HepG2 using 84522-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 84522-6-RR

    FC experiment of HeLa using 84522-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LBR Recombinant antibody

LBR Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84522-6-RR

产品说明书

CloneNo. 241864H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, C2C12 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LBR Ready-To-Use IHC Kit. LBR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84522-6-RR targets LBR in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LBR fusion protein Ag3088 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lamin B receptor
别名 Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1
计算分子量 615 aa, 71 kDa
观测分子量58 kDa
GenBank蛋白编号BC020079
基因名称 LBR
Gene ID (NCBI) 3930
RRIDAB_3672029
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14739
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

实验方案

Product Specific Protocols
WB protocol for LBR antibody 84522-6-RRDownload protocol
FC protocol for LBR antibody 84522-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols