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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated LGMN Monoclonal antibody

LGMN Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2B12

Cat no : CL488-67017

Print datasheet

Synonyms

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

验证数据展示

  • IF Staining of human kidney using CL488-67017

    IF Staining of human kidney using CL488-67017

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 LGMN antibody (CL488-67017, Clone: 1F2B12 ) at dilution of 1:200.

  • IF Staining of human kidney using CL488-67017

    IF Staining of human kidney using CL488-67017

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 LGMN antibody (CL488-67017, Clone: 1F2B12 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy LGMN Ready-To-Use IHC Kit. LGMN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67017-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67017 targets LGMN in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen LGMN fusion protein Ag7218 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name legumain
Synonyms AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC003061
Gene Symbol LGMN
Gene ID (NCBI) 5641
RRIDAB_2919406
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99538
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LGMN antibody CL488-67017Download protocol
Standard Protocols
Click here to view our Standard Protocols