验证数据展示
经过测试的应用
| Positive IF-P detected in | human kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67017 targets LGMN in IF-P applications and shows reactivity with Human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human |
| 免疫原 | LGMN fusion protein Ag7218 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | legumain |
| 别名 | AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1 |
| 计算分子量 | 49 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC003061 |
| 基因名称 | LGMN |
| Gene ID (NCBI) | 5641 |
| RRID | AB_2920072 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99538 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 LGMN antibody CL594-67017 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |