验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, SH-SY5Y cells, pig brain tissue, A431 cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LIMK1 Ready-To-Use IHC Kit. LIMK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
67974-1-Ig targets LIMK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Immunogen | LIMK1 fusion protein Ag28862 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | LIM domain kinase 1 |
| Synonyms | LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1 |
| Calculated Molecular Weight | 647 aa, 73 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC152982 |
| Gene Symbol | LIMK1 |
| Gene ID (NCBI) | 3984 |
| RRID | AB_3085042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P53667 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIMK1 antibody 67974-1-Ig | Download protocol |
| IHC protocol for LIMK1 antibody 67974-1-Ig | Download protocol |
| IF protocol for LIMK1 antibody 67974-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Tissue Cell B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway | ||
Zool Res PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior |
