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LIMK1 Monoclonal antibody

LIMK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67974-1-Ig

产品说明书

CloneNo. 2B11E9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA and More (1)

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, SH-SY5Y cells, pig brain tissue, A431 cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LIMK1 Ready-To-Use IHC Kit. LIMK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67974-1-Ig targets LIMK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human, mouse
免疫原 LIMK1 fusion protein Ag28862 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 LIM domain kinase 1
别名 LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1
计算分子量 647 aa, 73 kDa
观测分子量65 kDa
GenBank蛋白编号BC152982
基因名称 LIMK1
Gene ID (NCBI) 3984
RRIDAB_3085042
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP53667
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

实验方案

Product Specific Protocols
WB protocol for LIMK1 antibody 67974-1-IgDownload protocol
IHC protocol for LIMK1 antibody 67974-1-IgDownload protocol
IF protocol for LIMK1 antibody 67974-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Tissue Cell

B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway

Authors - Anjing Zhao
mouseWB,IF

Zool Res

PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior

Authors - Laidong Yu
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