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验证数据展示

  • FC experiment of HeLa using CL488-67974

    FC experiment of HeLa using CL488-67974

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated LIMK1 Monoclonal antibody (CL488-67974, Clone:2B11E9)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67974

    IF Staining of HeLa using CL488-67974

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 LIMK1 antibody (CL488-67974, Clone: 2B11E9 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated LIMK1 Monoclonal antibody

LIMK1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67974

Print datasheet

CloneNo. 2B11E9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC, FC (Intra)

Synonyms

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LIMK1 Ready-To-Use IHC Kit. LIMK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67974-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67974 targets LIMK1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen LIMK1 fusion protein Ag28862 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name LIM domain kinase 1
Synonyms LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1
Calculated Molecular Weight 647 aa, 73 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC152982
Gene Symbol LIMK1
Gene ID (NCBI) 3984
RRIDAB_3672967
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53667
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LIMK1 antibody CL488-67974Download protocol
FC protocol for CL Plus 488 LIMK1 antibody CL488-67974Download protocol
Standard Protocols
Click here to view our Standard Protocols