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验证数据展示

  • IF Staining of human prostate cancer using CL488-60344

    IF Staining of human prostate cancer using CL488-60344

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 LIN28 antibody (CL488-60344, Clone: 4F5G6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated LIN28A Monoclonal antibody

LIN28A Monoclonal Antibody for IF-P
Cat No. CL488-60344

产品说明书

CloneNo. 4F5G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF-P

别名

LIN28, Lin-28A, Lin 28A, LIN 28, CSDD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman prostate cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60344-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60344 targets LIN28A in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 LIN28A fusion protein Ag2312 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lin-28 homolog (C. elegans)
别名 LIN28, Lin-28A, Lin 28A, LIN 28, CSDD1
计算分子量 209 aa, 23 kDa
观测分子量 28 kDa
GenBank蛋白编号BC028566
基因名称 LIN28
Gene ID (NCBI) 79727
RRIDAB_2919251
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H9Z2
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LIN28A antibody CL488-60344Download protocol
Standard Protocols
Click here to view our Standard Protocols