CoraLite® Plus 488-conjugated LONP1 Recombinant antibody

LONP1 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240460E5

Cat no : CL488-83552-3

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Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53



经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83552-3-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83552-3 is LONP1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen LONP1 fusion protein Ag7196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53
Calculated Molecular Weight 106 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36776
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LONP1 antibody CL488-83552-3Download protocol
Standard Protocols
Click here to view our Standard Protocols