CoraLite® Plus 488-conjugated LONP1 Recombinant antibody
LONP1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240460E5
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 83552-3-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL488-83552-3 is LONP1 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human |
Immunogen | LONP1 fusion protein Ag7196 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | lon peptidase 1, mitochondrial |
Synonyms | Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53 |
Calculated Molecular Weight | 106 kDa |
GenBank Accession Number | BC000235 |
Gene Symbol | LONP1 |
Gene ID (NCBI) | 9361 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P36776 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 LONP1 antibody CL488-83552-3 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |