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LPCAT2 Monoclonal antibody

LPCAT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E6G1

Cat no : 68476-1-Ig

Print datasheet

Synonyms

Acyltransferase like 1, AYTL1, DKFZp686H22112, FLJ20481, LPC acyltransferase 2, LPCAT 2, LPCAT2, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 2

验证数据展示

  • WB analysis using 68476-1-Ig

    WB analysis using 68476-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68476-1-Ig (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inBxPC-3 cells, A375 cells, A549 cells, HEK-293 cells, HT-29 cells, Jurkat cells, HL-60 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68476-1-Ig targets LPCAT2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen LPCAT2 fusion protein Ag26420 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lysophosphatidylcholine acyltransferase 2
Synonyms Acyltransferase like 1, AYTL1, DKFZp686H22112, FLJ20481, LPC acyltransferase 2, LPCAT 2, LPCAT2, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC002472
Gene Symbol LPCAT2
Gene ID (NCBI) 54947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7L5N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPCAT2 (Lysophosphatidylcholine acyltransferase 2) is also named as AGPAT11 and AYTL1. Lipid droplet (LD) content of CRC cells positively correlates with the expression of lysophosphatidylcholine acyltransferase 2 (LPCAT2), an LD-localised enzyme supporting phosphatidylcholine synthesis (PMID: 29358673). Mitochondria play a significant role in ACSL4/LPCAT2-driven ferroptosis (PMID: 3762584). In response to LPS, LPCAT2, but not LPCAT1, rapidly associates with TLR4 and translocates to membrane lipid raft domains to regulate macrophage inflammatory gene expression (PMID: 32587324).

实验方案

Product Specific Protocols
WB protocol for LPCAT2 antibody 68476-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols