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验证数据展示

  • WB analysis using 68476-1-Ig

    WB analysis using 68476-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68476-1-Ig (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

LPCAT2 Monoclonal antibody

LPCAT2 Monoclonal Antibody for WB, ELISA
Cat No. 68476-1-Ig

产品说明书

CloneNo. 1E6G1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

Acyltransferase like 1, AYTL1, DKFZp686H22112, FLJ20481, LPC acyltransferase 2, LPCAT 2, LPCAT2, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, A375 cells, A549 cells, HEK-293 cells, HT-29 cells, Jurkat cells, HL-60 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy LPCAT2 Ready-To-Use IHC Kit. LPCAT2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68476-1-Ig targets LPCAT2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 LPCAT2 fusion protein Ag26420 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lysophosphatidylcholine acyltransferase 2
别名 Acyltransferase like 1, AYTL1, DKFZp686H22112, FLJ20481, LPC acyltransferase 2, LPCAT 2, LPCAT2, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 2
计算分子量 60 kDa
观测分子量 68 kDa
GenBank蛋白编号BC002472
基因名称 LPCAT2
Gene ID (NCBI) 54947
RRIDAB_3085187
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7L5N7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPCAT2 (Lysophosphatidylcholine acyltransferase 2) is also named as AGPAT11 and AYTL1. Lipid droplet (LD) content of CRC cells positively correlates with the expression of lysophosphatidylcholine acyltransferase 2 (LPCAT2), an LD-localised enzyme supporting phosphatidylcholine synthesis (PMID: 29358673). Mitochondria play a significant role in ACSL4/LPCAT2-driven ferroptosis (PMID: 3762584). In response to LPS, LPCAT2, but not LPCAT1, rapidly associates with TLR4 and translocates to membrane lipid raft domains to regulate macrophage inflammatory gene expression (PMID: 32587324).

实验方案

Product Specific Protocols
WB protocol for LPCAT2 antibody 68476-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols