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验证数据展示

  • WB analysis of pig brain using 66957-1-Ig

    WB analysis of pig brain using 66957-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 66957-1-Ig

    IHC staining of human heart using 66957-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 66957-1-Ig

    IHC staining of human heart using 66957-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66957-1-Ig

    IF Staining of HeLa using 66957-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LXN antibody (66957-1-Ig, Clone: 3E5A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66957-1-Ig

    FC experiment of HeLa using 66957-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LXN (66957-1-Ig, Clone:3E5A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LXN Monoclonal antibody

LXN Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66957-1-Ig

产品说明书

CloneNo. 3E5A5

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rabbit

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

TCI, Protein MUM, Latexin, ECI, 3E5A5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, A549 cells, HEK-293 cells, SH-SY5Y cells, rabbit brain tissue, HeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66957-1-Ig targets LXN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原 LXN fusion protein Ag27922 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 latexin
别名 TCI, Protein MUM, Latexin, ECI, 3E5A5
计算分子量 222 aa, 26 kDa
观测分子量 30 kDa
GenBank蛋白编号BC005346
基因名称 LXN
Gene ID (NCBI) 56925
RRIDAB_2882280
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BS40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.

实验方案

Product Specific Protocols
WB protocol for LXN antibody 66957-1-IgDownload protocol
IHC protocol for LXN antibody 66957-1-IgDownload protocol
IF protocol for LXN antibody 66957-1-IgDownload protocol
FC protocol for LXN antibody 66957-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols