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LXN Monoclonal antibody, PBS Only (Capture)

LXN Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, human

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E5A5

Cat no : 66957-1-PBS

Print datasheet

Synonyms

TCI, Protein MUM, Latexin, ECI, 3E5A5

验证数据展示

  • WB analysis of pig brain using 66957-1-Ig (same clone as 66957-1-PBS)

    WB analysis of pig brain using 66957-1-Ig (same clone as 66957-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

    IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

    IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

    IF Staining of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LXN antibody (66957-1-Ig, Clone: 3E5A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50023-1

    Sandwich ELISA standard curve of MP50023-1

    Sandwich ELISA standard curve ofMP50023-1, LXN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66957-1-PBS. Detection antibody: HRP-conjugated 66957-2-PBS. Standard: Ag27922. Range: 0.313-20 ng/mL.

  • FC experiment of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

    FC experiment of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LXN (66957-1-Ig, Clone:3E5A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

产品信息

66957-1-PBS targets LXN as part of a matched antibody pair:

MP50023-1: 66957-1-PBS capture and 66957-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity rabbit, pig, human
Immunogen LXN fusion protein Ag27922 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name latexin
Synonyms TCI, Protein MUM, Latexin, ECI, 3E5A5
Calculated Molecular Weight 222 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC005346
Gene Symbol LXN
Gene ID (NCBI) 56925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BS40
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.