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验证数据展示

  • FC experiment of human PBMCs using CL647-65283

    FC experiment of human PBMCs using CL647-65283

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human M-CSF (CL647-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65283

    FC experiment of human PBMCs using CL647-65283

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human M-CSF (CL647-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human M-CSF (CL647-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 647 Anti-Human M-CSF (3G12E6)

M-CSF Monoclonal Antibody for FC (Intra)
Cat No. CL647-65283

产品说明书

CloneNo. 3G12E6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

M-CSF, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Proteoglycan macrophage colony-stimulating factor, 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim, M CSF, Macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, MCSF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65283-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65283 targets M-CSF in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 M-CSF fusion protein HZ-1192 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 colony stimulating factor 1 (macrophage)
别名 M-CSF, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Proteoglycan macrophage colony-stimulating factor, 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim, M CSF, Macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, MCSF
计算分子量 60 kDa
GenBank蛋白编号BC021117
基因名称 M-CSF
Gene ID (NCBI) 1435
RRIDAB_3084818
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 M-CSF antibody CL647-65283Download protocol
Standard Protocols
Click here to view our Standard Protocols