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验证数据展示

  • FC experiment of human PBMCs using CL750-65283

    FC experiment of human PBMCs using CL750-65283

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human M-CSF (CL750-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL750-65283

    FC experiment of human PBMCs using CL750-65283

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human M-CSF (CL750-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human M-CSF (CL750-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 750 Anti-Human M-CSF (3G12E6)

M-CSF Monoclonal Antibody for FC (Intra)
Cat No. CL750-65283

Print datasheet

CloneNo. 3G12E6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Synonyms

M-CSF, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Proteoglycan macrophage colony-stimulating factor, 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim, M CSF, Macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, MCSF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65283-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-65283 targets M-CSF in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen M-CSF fusion protein HZ-1192 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name colony stimulating factor 1 (macrophage)
Synonyms M-CSF, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Proteoglycan macrophage colony-stimulating factor, 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim, M CSF, Macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, MCSF
Calculated Molecular Weight 60 kDa
GenBank Accession NumberBC021117
Gene Symbol M-CSF
Gene ID (NCBI) 1435
RRIDAB_3084885
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 M-CSF antibody CL750-65283Download protocol
Standard Protocols
Click here to view our Standard Protocols