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验证数据展示

  • IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

    IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAD2L2 antibody included
  • WB analysis of human brain using 12683-1-AP (same clone as 12683-1-PBS)

    WB analysis of human brain using 12683-1-AP (same clone as 12683-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

    IP experiment of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

    IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MAD2L2 Polyclonal antibody, PBS Only

MAD2L2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12683-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAD2L2 Ready-To-Use IHC Kit. MAD2L2 primary antibody included.

产品信息

12683-1-PBS targets MAD2L2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAD2L2 fusion protein Ag3373 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAD2 mitotic arrest deficient-like 2 (yeast)
别名
计算分子量 211 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC015244
基因名称 MAD2L2
Gene ID (NCBI) 10459
RRIDAB_2139530
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI95
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.