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  • Featured Product
  • KD/KO Validated

MAD2L2 Polyclonal antibody, PBS Only

MAD2L2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12683-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

    IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAD2L2 antibody included
  • WB analysis of human brain using 12683-1-AP (same clone as 12683-1-PBS)

    WB analysis of human brain using 12683-1-AP (same clone as 12683-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

    IP experiment of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

    IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAD2L2 Ready-To-Use IHC Kit. MAD2L2 primary antibody included.

产品信息

12683-1-PBS targets MAD2L2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAD2L2 fusion protein Ag3373 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Synonyms
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC015244
Gene Symbol MAD2L2
Gene ID (NCBI) 10459
RRIDAB_2139530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI95
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.