CoraLite® Plus 488-conjugated MAN1B1 Monoclonal antibody
MAN1B1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C9F10
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 68121-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68121 targets MAN1B1 in IF/ICC applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human |
| Immunogen | MAN1B1 fusion protein Ag28302 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mannosidase, alpha, class 1B, member 1 |
| Synonyms | ER mannosidase 1, ER alpha-1,2-mannosidase, ER alpha 1,2 mannosidase, Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, EC:3.2.1.113 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC002953 |
| Gene Symbol | MAN1B1 |
| Gene ID (NCBI) | 11253 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKM7 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MAN1B1 antibody CL488-68121 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |