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验证数据展示

  • IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2 antibody included
  • IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

  • FC experiment of Neuro-2a using 67015-1-Ig (same clone as 67015-1-PBS)

    FC experiment of Neuro-2a using 67015-1-Ig (same clone as 67015-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2 antibody included
  • IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2 antibody included
  • IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2 antibody included
  • IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), GFAP antibody (81063-1-RR, Clone: 4C6, red). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 GFAP antibody (CL594-16825, red), TDP-43 antibody (10782-2-AP, Magenta). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

MAP2 Monoclonal antibody, PBS Only

MAP2 Monoclonal Antibody for IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA
Cat No. 67015-1-PBS

产品说明书

CloneNo. 1C3E6
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA and More (1)

别名

1C3E6, MAP 2, MAP-2, MAP2A, MAP2B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.

发表文章中的应用

IFSee 1 publications below

产品信息

67015-1-PBS targets MAP2 in IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag11349

Product name: Recombinant human MAP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 213-559 aa of BC038857

Sequence: TSAGSTDRLPYSKSGNKDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVRILNKKIDFSKVQSRCGSKDNIKHSAGGGNVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 microtubule-associated protein 2
别名 1C3E6, MAP 2, MAP-2, MAP2A, MAP2B
计算分子量 200 kDa
GenBank蛋白编号BC038857
基因名称 MAP2
Gene ID (NCBI) 4133
RRIDAB_2882331
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11137
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

发表文章

SpeciesApplicationTitle
mouseIF

J Neuroinflammation

D-serine ameliorates cognitive deficits by preserving neuronal and synaptic function in experimental anti-NMDAR encephalitis.

Authors - Yu Yao
View Article
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