验证数据展示
经过测试的应用
| Positive IHC detected in | mouse brain tissue, rat cerebellum tissue, rat brain tissue, human brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive FC (Intra) detected in | Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67015-1-Ig targets MAP2 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | MAP2 fusion protein Ag11349 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 2 |
| 别名 | MAP2C, MAP2B, MAP2A, MAP-2, MAP 2 |
| 计算分子量 | 200 kDa |
| GenBank蛋白编号 | BC038857 |
| 基因名称 | MAP2 |
| Gene ID (NCBI) | 4133 |
| RRID | AB_2882331 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11137 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP2 antibody 67015-1-Ig | Download protocol |
| IF protocol for MAP2 antibody 67015-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury. | ||
Nat Chem Biol Tracking endogenous proteins based on RNA editing-mediated genetic code expansion | ||
Small Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect. | ||
Cell Death Dis ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis | ||
Cell Death Dis Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes |
