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验证数据展示

  • WB analysis of U-251 using 81063-1-RR

    WB analysis of U-251 using 81063-1-RR

    U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 81063-1-RR

    IHC staining of rat brain using 81063-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GFAP antibody included
  • IHC staining of rat brain using 81063-1-RR

    IHC staining of rat brain using 81063-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GFAP antibody included
  • IF Staining of rat brain using 81063-1-RR

    IF Staining of rat brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 81063-1-RR

    WB analysis using 81063-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 81063-1-RR

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 81063-1-RR

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of U-251 using 81063-1-RR

    WB analysis of U-251 using 81063-1-RR

    WB result of GFAP antibody (81063-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells.

  • IF Staining of mouse brain using 81063-1-RR

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of rat brain using 81063-1-RR

    IF Staining of rat brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, green).

  • Affinity and Kinetic Characterization of 81063-1-RR

    Affinity and Kinetic Characterization of 81063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human GFAP were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

GFAP Recombinant monoclonal antibody

GFAP Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 81063-1-RR

产品说明书

CloneNo. 4C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, ELISA

别名

4C6, glial fibrillary acidic protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse brain tissue
Positive IF-Fro detected inmouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4950-1:19800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81063-1-RR targets GFAP in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag10423

Product name: Recombinant human GFAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 83-432 aa of BC013596

Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glial fibrillary acidic protein
别名 4C6, glial fibrillary acidic protein
计算分子量 432 aa, 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC013596
基因名称 GFAP
Gene ID (NCBI) 2670
RRIDAB_2923699
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14136
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

实验方案

Product Specific Protocols
WB protocol for GFAP antibody 81063-1-RRDownload protocol
IHC protocol for GFAP antibody 81063-1-RRDownload protocol
IF protocol for GFAP antibody 81063-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols