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CoraLite® Plus 488-conjugated MAP2 Monoclonal antibody

MAP2 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3E6

Cat no : CL488-67015

Print datasheet

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

验证数据展示

  • FC experiment of Neuro-2a using CL488-67015

    FC experiment of Neuro-2a using CL488-67015

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MAP2 (CL488-67015, Clone:1C3E6) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of rat brain using CL488-67015

    IF Staining of rat brain using CL488-67015

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 MAP2 antibody (CL488-67015, Clone: 1C3E6 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67015-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67015 targets MAP2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MAP2 fusion protein Ag11349 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name microtubule-associated protein 2
Synonyms MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
Calculated Molecular Weight 200 kDa
GenBank Accession NumberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11137
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAP2 antibody CL488-67015Download protocol
FC protocol for CL Plus 488 MAP2 antibody CL488-67015Download protocol
Standard Protocols
Click here to view our Standard Protocols