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验证数据展示

  • FC experiment of Neuro-2a using CL594-67015

    FC experiment of Neuro-2a using CL594-67015

    1X10^6 Neuro-2a cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human MAP2 (CL594-67015, Clone:1C3E6) (red), or 0.2 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of rat brain using CL594-67015

    IF Staining of rat brain using CL594-67015

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 MAP2 antibody (CL594-67015, Clone: 1C3E6 ) at dilution of 1:200.

CoraLite®594-conjugated MAP2 Monoclonal antibody

MAP2 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-67015

产品说明书

CloneNo. 1C3E6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67015-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67015 targets MAP2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MAP2 fusion protein Ag11349 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 microtubule-associated protein 2
别名 MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
计算分子量 200 kDa
GenBank蛋白编号BC038857
基因名称 MAP2
Gene ID (NCBI) 4133
RRIDAB_2934755
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11137
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

实验方案

Product Specific Protocols
IF protocol for CL594 MAP2 antibody CL594-67015Download protocol
FC protocol for CL594 MAP2 antibody CL594-67015Download protocol
Standard Protocols
Click here to view our Standard Protocols