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验证数据展示

  • WB analysis using 84306-3-RR (same clone as 84306-3-PBS)

    WB analysis using 84306-3-RR (same clone as 84306-3-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84306-3-RR (MAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

  • WB analysis using 84306-3-RR (same clone as 84306-3-PBS)

    WB analysis using 84306-3-RR (same clone as 84306-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84306-3-RR (MAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01208-3

    Sandwich ELISA standard curve of MP01208-3

    Sandwich ELISA standard curve of MP01208-3, Human MAP2 Recombinant Matched Antibody Pair - PBS only. 84306-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11580. 84306-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP01208-3

    Sample test of MP01208-3

    The mean MAP2 concentration was determined to be 479.14 pg/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load.

  • IHC staining of mouse cerebellum using 84306-3-RR (same clone as 84306-3-PBS)

    IHC staining of mouse cerebellum using 84306-3-RR (same clone as 84306-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84306-3-RR (MAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2 antibody included
  • IHC staining of mouse cerebellum using 84306-3-RR (same clone as 84306-3-PBS)

    IHC staining of mouse cerebellum using 84306-3-RR (same clone as 84306-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84306-3-RR (MAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2 antibody included
  • IF Staining of rat brain using 84306-3-RR (same clone as 84306-3-PBS)

    IF Staining of rat brain using 84306-3-RR (same clone as 84306-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).MAP2 antibody (84306-3-RR, Clone: 241653E9,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84306-3-RR (same clone as 84306-3-PBS)

    IF Staining of mouse brain using 84306-3-RR (same clone as 84306-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-3-RR, Clone: 241653E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01208-1

    Cytometric bead array standard curve of MP01208-1

    Cytometric bead array standard curve of MP01208-1, MAP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84306-2-PBS. Detection antibody: 84306-3-PBS. Standard: Ag11580. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01208-2

    Cytometric bead array standard curve of MP01208-2

    Cytometric bead array standard curve of MP01208-2, MAP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84306-1-PBS. Detection antibody: 84306-3-PBS. Standard: Ag11580. Range: 0.625-80 ng/mL.

MAP2 Recombinant antibody, PBS Only (Detector)

MAP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84306-3-PBS

Print datasheet

CloneNo. 241653E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.

产品信息

84306-3-PBS targets MAP2 as part of a matched antibody pair:

MP01208-1: 84306-2-PBS capture and 84306-3-PBS detection (validated in Cytometric bead array)

MP01208-2: 84306-1-PBS capture and 84306-3-PBS detection (validated in Cytometric bead array)

MP01208-3: 84306-5-PBS capture and 84306-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF-P, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen MAP2 fusion protein Ag11580 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name microtubule-associated protein 2
Synonyms MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
Calculated Molecular Weight 200 kDa
Observed Molecular Weight280-300 kDa, 70-85 kDa
GenBank Accession NumberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11137
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.