MAP2 Recombinant antibody

MAP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 84306-3-RR

产品说明书

CloneNo. 241653E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, ELISA

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF-Fro detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84306-3-RR targets MAP2 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAP2 fusion protein Ag11580 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein 2
别名 MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
计算分子量 200 kDa
观测分子量280-300 kDa, 70-85 kDa
GenBank蛋白编号BC038857
基因名称 MAP2
Gene ID (NCBI) 4133
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11137
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

实验方案

Product Specific Protocols
WB protocol for MAP2 antibody 84306-3-RRDownload protocol
IHC protocol for MAP2 antibody 84306-3-RRDownload protocol
IF protocol for MAP2 antibody 84306-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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