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MAP2 Recombinant antibody

MAP2 Uni-rAbTM Recombinant Antibody for IF-P, IF-Fro, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IF-Fro, ELISA

Conjugate

Unconjugated

CloneNo.

241653E9

Cat no : 84306-3-RR

Print datasheet

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

验证数据展示

  • IF Staining of rat brain using 84306-3-RR

    IF Staining of rat brain using 84306-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).MAP2 antibody (84306-3-RR, Clone: 241653E9,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 84306-3-RR

    IF Staining of mouse brain using 84306-3-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-3-RR, Clone: 241653E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF-P detected inrat brain tissue
Positive IF-Fro detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy MAP2 Ready-To-Use IHC Kit. MAP2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84306-3-RR targets MAP2 in IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IF-Fro, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAP2 fusion protein Ag11580 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name microtubule-associated protein 2
Synonyms MAP2C, MAP2B, MAP2A, MAP-2, MAP 2
Calculated Molecular Weight 200 kDa
GenBank Accession NumberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

实验方案

Product Specific Protocols
IF protocol for MAP2 antibody 84306-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols