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验证数据展示

  • IF Staining of HEK-293 using CL488-12202

    IF Staining of HEK-293 using CL488-12202

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 MATR3 antibody (CL488-12202) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL488-12202

    FC experiment of SH-SY5Y using CL488-12202

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MATR3 (CL488-12202) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated MATR3 Polyclonal antibody

MATR3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12202

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Matrin-3, matrin 3, KIAA0723

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy MATR3 Ready-To-Use IHC Kit. MATR3 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12202 targets MATR3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MATR3 fusion protein Ag2843 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrin 3
别名 Matrin-3, matrin 3, KIAA0723
计算分子量 847 aa, 95 kDa
观测分子量125 kDa
GenBank蛋白编号BC015031
基因名称 MATR3
Gene ID (NCBI) 9782
RRIDAB_3083920
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43243
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MATR3, known as KIAA0723, is a 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa prtein as result from PMID: 28977530.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MATR3 antibody CL488-12202Download protocol
Standard Protocols
Click here to view our Standard Protocols