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MAdCAM1 Monoclonal antibody, PBS Only

MAdCAM1 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A12E8

Cat no : 66594-1-PBS

Print datasheet

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1

验证数据展示

  • IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

    IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAdCAM1 antibody included
  • IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

    IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAdCAM1 antibody included
  • IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAdCAM1 Ready-To-Use IHC Kit. MAdCAM1 primary antibody included.

产品信息

66594-1-PBS targets MAdCAM1 in IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MAdCAM1 fusion protein Ag16184 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mucosal vascular addressin cell adhesion molecule 1
Synonyms hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1
Calculated Molecular Weight 406 aa, 43 kDa
GenBank Accession NumberBC142629
Gene Symbol MADCAM1
Gene ID (NCBI) 8174
RRIDAB_2881954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13477
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.