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MCL1 Recombinant antibody, PBS Only

MCL1 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7

Cat No : 82695-2-PBS

Print datasheet

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

验证数据展示

  • WB analysis using 82695-2-RR (same clone as 82695-2-PBS)

    WB analysis using 82695-2-RR (same clone as 82695-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82695-2-RR (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 82695-2-RR (same clone as 82695-2-PBS)

    IF Staining of MCF-7 using 82695-2-RR (same clone as 82695-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (82695-2-RR, Clone: 1C7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

产品信息

82695-2-PBS targets MCL1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MCL1 fusion protein Ag10397 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Synonyms EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM
Calculated Molecular Weight 350 aa, 37 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC107735
Gene Symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07820
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)