• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MCL1 Recombinant antibody, PBS Only

MCL1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7

Cat no : 82695-2-PBS

Print datasheet

Synonyms

Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3

验证数据展示

  • WB analysis using 82695-2-RR (same clone as 82695-2-PBS)

    WB analysis using 82695-2-RR (same clone as 82695-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82695-2-RR (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82695-2-RR (same clone as 82695-2-PBS)

    IHC staining of human ovarian cancer using 82695-2-RR (same clone as 82695-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-2-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • IHC staining of human ovarian cancer using 82695-2-RR (same clone as 82695-2-PBS)

    IHC staining of human ovarian cancer using 82695-2-RR (same clone as 82695-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-2-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • IF Staining of MCF-7 using 82695-2-RR (same clone as 82695-2-PBS)

    IF Staining of MCF-7 using 82695-2-RR (same clone as 82695-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (82695-2-RR, Clone: 1C7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

产品信息

82695-2-PBS targets MCL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MCL1 fusion protein Ag10397 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Synonyms Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3
Calculated Molecular Weight 350 aa, 37 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC107735
Gene Symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07820
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)