• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MCM2 Recombinant antibody

MCM2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241968D3

Cat no : 84511-2-RR

Print datasheet

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

验证数据展示

  • WB analysis using 84511-2-RR

    WB analysis using 84511-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84511-2-RR (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84511-2-RR

    IF Staining of HeLa using 84511-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-2-RR, Clone: 241968D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84511-2-RR

    FC experiment of HeLa using 84511-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM2 Recombinant antibody (84511-2-RR, Clone:241968D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84511-2-RR

    Affinity and Kinetic Characterization of 84511-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84511-2-RR against Human MCM2 were performed. The affinity constant is 0.453 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, Jurkat cells, A431 cells
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84511-2-RR targets MCM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 116-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

实验方案

Product Specific Protocols
WB protocol for MCM2 antibody 84511-2-RRDownload protocol
IF protocol for MCM2 antibody 84511-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols